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biolock biotin blocking solution  (IBA Lifesciences)


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    Structured Review

    IBA Lifesciences biolock biotin blocking solution
    Biolock Biotin Blocking Solution, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 96/100, based on 266 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biolock+biotin+blocking+solution/BioLock/pmc13639993-274-8-12
    Average 96 stars, based on 266 article reviews
    biolock biotin blocking solution - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Protein Purification:

    Article Title: Structural basis of GAIN domain autoproteolysis and cleavage-resistance in the adhesion G-protein coupled receptors.
    Article Snippet: .. Protein purification - 1 mL BioLock biotin blocking solution (cat# 2-0205-050, IBA Lifesciences) was added per 1.5 L of conditioned medium and incubated for 2 h gently stirring at 4°C. .. The medium was then centrifuged for 30 min at 18,600 g and the supernatant filtered through a 0.22 μm polyethersulfone membrane (cat# 514-0332P, Avantor).

    Blocking Assay:

    Article Title: Structural basis of GAIN domain autoproteolysis and cleavage-resistance in the adhesion G-protein coupled receptors.
    Article Snippet: .. Protein purification - 1 mL BioLock biotin blocking solution (cat# 2-0205-050, IBA Lifesciences) was added per 1.5 L of conditioned medium and incubated for 2 h gently stirring at 4°C. .. The medium was then centrifuged for 30 min at 18,600 g and the supernatant filtered through a 0.22 μm polyethersulfone membrane (cat# 514-0332P, Avantor).

    Article Title: Structural basis of GAIN domain autoproteolysis and cleavage-resistance in the adhesion G-protein coupled receptors
    Article Snippet: .. 1 mL BioLock biotin blocking solution (cat# 2-0205-050, IBA Lifesciences) was added per 1.5 L of conditioned medium and incubated for 2 h, gently stirring at 4 °C. .. The medium was then centrifuged for 30 min at 18,600 g and the supernatant filtered through a 0.22 μm polyethersulfone membrane (cat# 514-0332 P, Avantor).

    Article Title: Integrative structural analysis of the human LRP2-LRPAP1 complex reveals multiple regulatory sites.
    Article Snippet: .. The supernatant was supplemented with 1/50 volume of BioLock Biotin blocking solution (IBA Lifesciences), 0.3 μM aprotinin, 1 μM pepstatin, 1 μM leupeptin, 1 mM benzamidine and 1 mM PMSF, and loaded onto a StrepTrap XT prepacked chromatography column (Cytiva). ..

    Article Title: HCF1 orchestrates O-GlcNAcylation and affinity-dependent transcription through extended molecular determinants and register-shifted binding
    Article Snippet: .. Cells were resuspended in lysis buffer containing 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton-X100, 1 mM DTT, cOmplete EDTA-free Protease Inhibitor Cocktail (Roche), a PhosSTOP tablet (Merck), and Biolock Biotin blocking solution (IBA). ..

    Article Title: Affinity-matured B cell responses neutralizing type-I interferons underlie severe viral infections
    Article Snippet: After 4–5 days of induction, the stably transfected S2 cell supernatants were harvested and concentrated to approximately 50 ml with the Vivaflow 200 ultrafiltration system with a 10 kDa cutoff (Sartorius). .. The scFv supernatants were then supplemented with 0.24% BioLock Biotin blocking solution (IBA) and 0.1 M Tris-HCl pH 8.0 and centrifuged for 30 minutes at 20,000 × g . .. For the scFvs, the process began with affinity purification on StrepTactin HP resin (Cytiva) based on binding of the double-strep tag.

    Article Title: Ubiquitinome dynamics and regulation by DUB2 during Leishmania differentiation
    Article Snippet: .. 2 μl of BioLock Biotin blocking solution (IBA Lifescience) per mL of HOMEM was added in a log-phase promastigote cell culture for 16 h. Cells were pelleted (1,000 x g , 10 min, RT), washed with filtered PBS (Phosphate Buffered Saline), harvested (1,000 x g , 10 min, RT), and resuspended with fresh HOMEM at a cell density of 5 x 10 6 cells mL -1 .For in vivo biotin labelling, biotin solution (Merck) was added to a log-phase (BioLock-treated) promastigote cell culture at a final concentration of 500 μM for 30 min at 25°C. ..

    Article Title: A kinetic ruler controls mRNA poly(A) tail length
    Article Snippet: .. Frozen pellet from 1 l culture was thawed in lysis buffer (50 mM Hepes-NaOH, pH 8.0, 300 mM NaCl, 1 mM TCEP) supplemented with cOmplete EDTA-free protease inhibitor cocktails (Sigma-Aldrich), and 1 ml BioLock biotin blocking solution (IBA Lifesciences). ..

    Incubation:

    Article Title: Structural basis of GAIN domain autoproteolysis and cleavage-resistance in the adhesion G-protein coupled receptors.
    Article Snippet: .. Protein purification - 1 mL BioLock biotin blocking solution (cat# 2-0205-050, IBA Lifesciences) was added per 1.5 L of conditioned medium and incubated for 2 h gently stirring at 4°C. .. The medium was then centrifuged for 30 min at 18,600 g and the supernatant filtered through a 0.22 μm polyethersulfone membrane (cat# 514-0332P, Avantor).

    Article Title: Structural basis of GAIN domain autoproteolysis and cleavage-resistance in the adhesion G-protein coupled receptors
    Article Snippet: .. 1 mL BioLock biotin blocking solution (cat# 2-0205-050, IBA Lifesciences) was added per 1.5 L of conditioned medium and incubated for 2 h, gently stirring at 4 °C. .. The medium was then centrifuged for 30 min at 18,600 g and the supernatant filtered through a 0.22 μm polyethersulfone membrane (cat# 514-0332 P, Avantor).

    Chromatography:

    Article Title: Integrative structural analysis of the human LRP2-LRPAP1 complex reveals multiple regulatory sites.
    Article Snippet: .. The supernatant was supplemented with 1/50 volume of BioLock Biotin blocking solution (IBA Lifesciences), 0.3 μM aprotinin, 1 μM pepstatin, 1 μM leupeptin, 1 mM benzamidine and 1 mM PMSF, and loaded onto a StrepTrap XT prepacked chromatography column (Cytiva). ..

    Lysis:

    Article Title: HCF1 orchestrates O-GlcNAcylation and affinity-dependent transcription through extended molecular determinants and register-shifted binding
    Article Snippet: .. Cells were resuspended in lysis buffer containing 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton-X100, 1 mM DTT, cOmplete EDTA-free Protease Inhibitor Cocktail (Roche), a PhosSTOP tablet (Merck), and Biolock Biotin blocking solution (IBA). ..

    Article Title: A kinetic ruler controls mRNA poly(A) tail length
    Article Snippet: .. Frozen pellet from 1 l culture was thawed in lysis buffer (50 mM Hepes-NaOH, pH 8.0, 300 mM NaCl, 1 mM TCEP) supplemented with cOmplete EDTA-free protease inhibitor cocktails (Sigma-Aldrich), and 1 ml BioLock biotin blocking solution (IBA Lifesciences). ..

    Protease Inhibitor:

    Article Title: HCF1 orchestrates O-GlcNAcylation and affinity-dependent transcription through extended molecular determinants and register-shifted binding
    Article Snippet: .. Cells were resuspended in lysis buffer containing 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton-X100, 1 mM DTT, cOmplete EDTA-free Protease Inhibitor Cocktail (Roche), a PhosSTOP tablet (Merck), and Biolock Biotin blocking solution (IBA). ..

    Article Title: A kinetic ruler controls mRNA poly(A) tail length
    Article Snippet: .. Frozen pellet from 1 l culture was thawed in lysis buffer (50 mM Hepes-NaOH, pH 8.0, 300 mM NaCl, 1 mM TCEP) supplemented with cOmplete EDTA-free protease inhibitor cocktails (Sigma-Aldrich), and 1 ml BioLock biotin blocking solution (IBA Lifesciences). ..

    Cell Culture:

    Article Title: Ubiquitinome dynamics and regulation by DUB2 during Leishmania differentiation
    Article Snippet: .. 2 μl of BioLock Biotin blocking solution (IBA Lifescience) per mL of HOMEM was added in a log-phase promastigote cell culture for 16 h. Cells were pelleted (1,000 x g , 10 min, RT), washed with filtered PBS (Phosphate Buffered Saline), harvested (1,000 x g , 10 min, RT), and resuspended with fresh HOMEM at a cell density of 5 x 10 6 cells mL -1 .For in vivo biotin labelling, biotin solution (Merck) was added to a log-phase (BioLock-treated) promastigote cell culture at a final concentration of 500 μM for 30 min at 25°C. ..

    Saline:

    Article Title: Ubiquitinome dynamics and regulation by DUB2 during Leishmania differentiation
    Article Snippet: .. 2 μl of BioLock Biotin blocking solution (IBA Lifescience) per mL of HOMEM was added in a log-phase promastigote cell culture for 16 h. Cells were pelleted (1,000 x g , 10 min, RT), washed with filtered PBS (Phosphate Buffered Saline), harvested (1,000 x g , 10 min, RT), and resuspended with fresh HOMEM at a cell density of 5 x 10 6 cells mL -1 .For in vivo biotin labelling, biotin solution (Merck) was added to a log-phase (BioLock-treated) promastigote cell culture at a final concentration of 500 μM for 30 min at 25°C. ..

    In Vivo:

    Article Title: Ubiquitinome dynamics and regulation by DUB2 during Leishmania differentiation
    Article Snippet: .. 2 μl of BioLock Biotin blocking solution (IBA Lifescience) per mL of HOMEM was added in a log-phase promastigote cell culture for 16 h. Cells were pelleted (1,000 x g , 10 min, RT), washed with filtered PBS (Phosphate Buffered Saline), harvested (1,000 x g , 10 min, RT), and resuspended with fresh HOMEM at a cell density of 5 x 10 6 cells mL -1 .For in vivo biotin labelling, biotin solution (Merck) was added to a log-phase (BioLock-treated) promastigote cell culture at a final concentration of 500 μM for 30 min at 25°C. ..

    Concentration Assay:

    Article Title: Ubiquitinome dynamics and regulation by DUB2 during Leishmania differentiation
    Article Snippet: .. 2 μl of BioLock Biotin blocking solution (IBA Lifescience) per mL of HOMEM was added in a log-phase promastigote cell culture for 16 h. Cells were pelleted (1,000 x g , 10 min, RT), washed with filtered PBS (Phosphate Buffered Saline), harvested (1,000 x g , 10 min, RT), and resuspended with fresh HOMEM at a cell density of 5 x 10 6 cells mL -1 .For in vivo biotin labelling, biotin solution (Merck) was added to a log-phase (BioLock-treated) promastigote cell culture at a final concentration of 500 μM for 30 min at 25°C. ..

    other:

    Article Title: Targeted bacteriophage T4 nanoparticles reverse HIV-1 latency in human T cell line models
    Article Snippet: RNA was isolated using Direct-Zol RNA MiniPrep Plus (Zymo Research, Irvine, CA) and cDNA was synthesized by using iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA).



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